Application
Immunocytochemistry of CXCL12 in HeLa cells with CXCL12 antibody at 5 μg/mL.Application
Western blot analysis of CXCL12 in HeLa cell lysate with CXCL12 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | CXCL12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CXCL12 Antibody: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, SDF1A, SDF1B, Stromal cell-derived factor 1, C-X-C motif chemokine 12, SDF-1 |
| Product description | The CXCL12 protein, also known as SDF1, is a stromal cell-derived alpha chemokine member of the intercrine family. CXCL12 functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4 (CXCR4) and CXCR7, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis (reviewed in 1). Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections (2).1) Timotijevic G, Mostarica Stojkovic M, and Miljkovic D. CXCL12: role in neuroinflammation. Int. J. Biochem. Cell Biol. 2012; 44:838-41. |
| Immunogen | CXCL12 antibody was raised against a 16 amino acid peptide near the center of human CXCL12 . The immunogen is located within the last 50 amino acids of CXCL12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| CXCL12 antibody can be used for detection of CXCL12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL12 |
|---|---|
| Alternative Names | CXCL12 Antibody: IRH PBSF SDF1 TLSF TPAR1 SCYB12 SDF1A SDF1B Stromal cell-derived factor 1 C-X-C motif chemokine 12 SDF-1 |
| Molecular Weight(MW) | Predicted: 15 kDa Observed: 18 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | CXCL12 antibody is human and mouse reactive. Multiple isoforms of CXCL12 are known to exist. |
| Entrez Gene | 6387 |
|---|---|
| Protein Accession | NP_001171605 |
Application
Immunocytochemistry of CXCL12 in HeLa cells with CXCL12 antibody at 5 μg/mL.
Application
Western blot analysis of CXCL12 in HeLa cell lysate with CXCL12 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | CXCL12 antibody can be used for detection of CXCL12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CXCL12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CXCL12 antibody is supplied in PBS containing 0.02% sodium azide. |
