Application
Immunocytochemistry of CXCR3 in HepG2 cells with CXCR3 antibody at 5 μg/mL.Application
Western blot analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of CXCR3 in HepG2 cells with CXCR3 antibody at 20 μg/mL.| Product Name | CXCR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The CXCR3 protein is a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-gamma), CXCL10/IP10 (interferon-gamma-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell alpha-chemoattractant) (reviewed in 1). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration (2). Similar to other chemokine receptors such as CXCR4, CXCR3 has been implicated in several malignancies as a biomarker of tumor behavior as well as a potential therapeutic target (3).1) Groom JR and Luster AD. CXCR3 ligands: redundant, collaborative and antagonistic functions. Immunol. Cell Biol. 2011; 89:207-15. |
| Immunogen | CXCR3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human CXCR3 . The immunogen is located within the last 50 amino acids of CXCR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| CXCR3 antibody can be used for detection of CXCR3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCR3 |
|---|---|
| Alternative Names | CXCR3 Antibody: GPR9 MigR CD182 CD183 Mig-R CKR-L2 CMKAR3 IP10-R GPR9 C-X-C chemokine receptor type 3 CXC-R3 |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 43 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Tissue Specificity | CXCR3 antibody is human, mouse and rat reactive. At least two isoforms of CXCR3 are known to exist; this antibody will detect both isoforms. |
| Entrez Gene | 2833 |
|---|---|
| Protein Accession | NP_001136269 |
Application
Immunocytochemistry of CXCR3 in HepG2 cells with CXCR3 antibody at 5 μg/mL.
Application
Western blot analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of CXCR3 in HepG2 cells with CXCR3 antibody at 20 μg/mL.| Application Notes | CXCR3 antibody can be used for detection of CXCR3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CXCR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CXCR3 antibody is supplied in PBS containing 0.02% sodium azide. |
