Proteins that contain a CXXC motif within their DNA-binding domain, such as CXXC1, recognize CpG sequences and regulate gene expression. Proteins that contain a CXXC motif within their DNA-binding domain, such as CXXC1, recognize CpG sequences and regulate gene expression (Carlone and Skalnik, 2001 [PubMed 11604496]).1) Ansari, K.I., (2008) Biochim. Biophys. Acta 1779 (1), 66-73.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CXXC1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CXXC1 antibody can be used for detection of CXXC1 by ELISA at 1:62500. CXXC1 antibody can be used for detection of CXXC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human THP-1 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1208 - THP-1 Cell Lysate
Application Notes
CXXC1 antibody can be used for detection of CXXC1 by ELISA at 1:62500. CXXC1 antibody can be used for detection of CXXC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CXXC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.