Application
Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | Cyclin H (phospho Thr315) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.1) Maekelae T.P., Nature 371:254-257(1994). |
| Immunogen | Cyclin H (phospho Thr315) antibody was raised against a peptide sequence around phosphorylation site of threonine 315 (E-W-T (p)-D-D) derived from Human Cyclin H . |
| Modification | p-Thr315 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNH |
|---|---|
| Alternative Names | cyclin H p34 p37 MO15-associated protein |
| Molecular Weight(MW) | 36 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315. |
| Entrez Gene | 902 |
|---|---|
| Protein Accession | NP_001230.1 |
Application
Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
