Application
Western blot analysis of Cyclin O in mouse bladder tissue lysate with Cyclin O antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Cyclin O Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.1) Roig MB, Roset R, Ortet L, et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Diff. 2009; 16:230-43. |
| Immunogen | Cyclin O antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Cyclin O. The immunogen is located within the first 50 amino acids of Cyclin O. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNO |
|---|---|
| Alternative Names | Cyclin O Antibody: CCNU UDG2 Cyclin-O |
| Molecular Weight(MW) | 39 kDa |
| Tissue Specificity | At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms. |
Application
Western blot analysis of Cyclin O in mouse bladder tissue lysate with Cyclin O antibody at (A) 1 and (B) 2 μg/mL.| Application Notes | Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | Cyclin O antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Cyclin O Antibody is supplied in PBS containing 0.02% sodium azide. |
