Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.Application
Antibody used in IHC on Human kidney at 1:100.| Product Name | CYP1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP1A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. CYP1A1 has been associated with lung cancer risk. This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Ikeda, S., (2008) Am. J. Gastroenterol. 103 (6), 1476-1487. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYP1A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CYP1A1 antibody can be used for detection of CYP1A1 by ELISA at 1:62500. CYP1A1 antibody can be used for detection of CYP1A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP1A1 |
|---|---|
| Alternative Names | CYP1A1 AHH AHRR CP11 CYP1 P1-450 P450-C P450DX |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney at 1:100.| Application Notes | CYP1A1 antibody can be used for detection of CYP1A1 by ELISA at 1:62500. CYP1A1 antibody can be used for detection of CYP1A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CYP1A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
