Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Product Name | CYP27A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP27A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Szlago, M., (2008) Neurology 70 (5), 402-404. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYP27A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CYP27A1 antibody can be used for detection of CYP27A1 by ELISA at 1:62500. CYP27A1 antibody can be used for detection of CYP27A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP27A1 |
|---|---|
| Alternative Names | CYP27A1 CP27 CTX CYP27 |
| Molecular Weight(MW) | 57 kDa |
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Application Notes | CYP27A1 antibody can be used for detection of CYP27A1 by ELISA at 1:62500. CYP27A1 antibody can be used for detection of CYP27A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
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| Form | Liquid |
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| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CYP27A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
