Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | CYP2C9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP2C9 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that CYP2C9 gene is polymorphic.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Mohammed (er) Br. J. Pharmacol. (2008) In press. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYP2C9. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CYP2C9 antibody can be used for detection of CYP2C9 by ELISA at 1:312500. CYP2C9 antibody can be used for detection of CYP2C9 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP2C9 |
|---|---|
| Alternative Names | CYP2C9 CPC9 CYP2C CYP2C10 MGC149605 MGC88320 P450 MP-4 P450 PB-1 P450IIC9 CYPIIC9 |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Application Notes | CYP2C9 antibody can be used for detection of CYP2C9 by ELISA at 1:312500. CYP2C9 antibody can be used for detection of CYP2C9 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CYP2C9 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
