Application
Confocal immunofluorescent analysis of CYP3A43 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CYP3A43 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.1) Domanski T.L., Finta C.Mol. Pharmacol. 59:386-392(2001) |
| Immunogen | This CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP3A43. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CYP3A43 |
|---|---|
| Alternative Names | Cytochrome P450 3A43 CYP3A43 |
| Molecular Weight(MW) | 58 kDa |
Application
Confocal immunofluorescent analysis of CYP3A43 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
