CYP4A22 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 1p33.1) Bellamine, A., (2003) Arch. Biochem. Biophys. 409 (1), 221-227.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYP4A22.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
CYP4A22 antibody can be used for detection of CYP4A22 by ELISA at 1:312500. CYP4A22 antibody can be used for detection of CYP4A22 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application Notes
CYP4A22 antibody can be used for detection of CYP4A22 by ELISA at 1:312500. CYP4A22 antibody can be used for detection of CYP4A22 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CYP4A22 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.