Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of DAP Kinase 1 (DAPK1) Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Antibody is used in Western blot to detect DAPK1 in mouse liver tissue lysate.| Product Name | DAPK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death (apoptosis). DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.1) Shohat, G., et al., J. Biol. Chem. 276(50):47460-47467 (2001). |
| Immunogen | This DAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1360-1389 amino acids from the C-terminal region of human DAPK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | DAPK1 |
|---|---|
| Alternative Names | Death-associated protein kinase 1 DAP kinase 1 DAPK1 DAPK |
| Molecular Weight(MW) | 160 kDa |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of DAP Kinase 1 (DAPK1) Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Antibody is used in Western blot to detect DAPK1 in mouse liver tissue lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
