Application
Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 μg/mL.Application
Immunohistochemistry of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 5 μg/mL.Application
Immunofluorescence of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 20 μg/mL.Application
Western blot analysis of DCLK1 in human brain tissue lysate with DCLK1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | DCLK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DCLK1 Antibody: DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.1) Sossey-Alaoui K and Srivastava AK. DCAMKL1, a brain specific transmembrane protein on 13q12.3 that is similar to doublecortin (DCX), Genomics1999; 56:121-6. |
| Immunogen | DCLK1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK1. The immunogen is located within the last 50 amino acids of DCLK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| DCLK1 antibody can be used for detection of DCLK1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCLK1 |
|---|---|
| Alternative Names | DCLK1 Antibody: CL1 DCLK CLICK1 DCDC3A DCAMKL1 KIAA0369 Doublecortin domain-containing protein 3A |
Application
Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 μg/mL.
Application
Immunohistochemistry of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 5 μg/mL.
Application
Immunofluorescence of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 20 μg/mL.
Application
Western blot analysis of DCLK1 in human brain tissue lysate with DCLK1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | DCLK1 antibody can be used for detection of DCLK1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | DCLK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DCLK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
