Application
Immunocytochemistry of DcR1 in HeLa cells with DcR1 antibody at 10 μg/mL.Application
Immunofluorescence of DcR1 in Hela cells with DcR1 antibody at 20 μg/mL.Application
Western blot analysis of DcR1 in HeLa whole cell lysate with DcR1 antibody (ED) at 1:500 dilution.| Product Name | DcR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DcR1 Antibody: LIT, DCR1, TRID, CD263, TRAILR3, TRAIL-R3, DCR1-TNFR, LIT, UNQ321/PRO366, Tumor necrosis factor receptor superfamily member 10C, Decoy TRAIL receptor without death domain, DcR1 |
| Product description | DcR1 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.1) Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113 |
| Immunogen | DcR1 antibody was raised against a peptide corresponding to amino acids in a extracellular domain of human DcR1 precursor. The immunogen is located within amino acids 140 - 190 of DcR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| DcR1 antibody can be used for detection of DcR1 by Western blot 0.5 μg/mL. An approximate 65 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF10C |
|---|---|
| Alternative Names | DcR1 Antibody: LIT DCR1 TRID CD263 TRAILR3 TRAIL-R3 DCR1-TNFR LIT UNQ321/PRO366 Tumor necrosis factor receptor superfamily member 10C Decoy TRAIL receptor without death domain DcR1 |
| Molecular Weight(MW) | 65 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of DcR1 in HeLa cells with DcR1 antibody at 10 μg/mL.
Application
Immunofluorescence of DcR1 in Hela cells with DcR1 antibody at 20 μg/mL.
Application
Western blot analysis of DcR1 in HeLa whole cell lysate with DcR1 antibody (ED) at 1:500 dilution.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | DcR1 antibody can be used for detection of DcR1 by Western blot 0.5 μg/mL. An approximate 65 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DcR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DcR1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM276808