Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | DDX21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX21, GUA, GURDB, RH-II/GU, RH-II/GuA |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.1) Yang, H., et al., (2005) FEBS J. 272 (15), 3788-3802. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX21. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DDX21 antibody can be used for detection of DDX21 by ELISA at 1:12500. DDX21 antibody can be used for detection of DDX21 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX21 |
|---|---|
| Alternative Names | DDX21 GUA GURDB RH-II/GU RH-II/GuA |
| Molecular Weight(MW) | 86 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DDX21 antibody can be used for detection of DDX21 by ELISA at 1:12500. DDX21 antibody can be used for detection of DDX21 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDX21 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
