Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
DDX27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | DDX27 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27, DDX27, RHLP |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].1) Deloukas, P., et al. Nature 414(6866):865-871(2001) |
| Immunogen | This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-796 amino acids from the C-terminal region of human DDX27. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX27 |
|---|---|
| Alternative Names | Probable ATP-dependent RNA helicase DDX27 DEAD box protein 27 DDX27 RHLP |
| Molecular Weight(MW) | 90 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
DDX27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
