Application
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 μg/mL.Application
Immunofluorescence of DEDAF in mouse liver tissue with DEDAF antibody at 20 μg/ml.Application
Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 μg/mL.Application
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 μg/mL.Application
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 μg /ml.| Product Name | DEDAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DEDAF Antibody: AAP1, DEDAF, YEAF1, RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1 |
| Product description | DEDAF Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.1) Zheng L, Schickling O, Peter ME, et al. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J. Biol. Chem. 2001; 276:31945-52. |
| Immunogen | DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF. The immunogen is located within the last 50 amino acids of DEDAF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RYBP |
|---|---|
| Alternative Names | DEDAF Antibody: AAP1 DEDAF YEAF1 RING1 and YY1-binding protein Apoptin-associating protein 1 APAP-1 |
| Molecular Weight(MW) | 32 kDa |
| Sequence Similarities | 4) Cat. No. 17-003 - A549 Cell Slide |
Application
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 μg/mL.
Application
Immunofluorescence of DEDAF in mouse liver tissue with DEDAF antibody at 20 μg/ml.
Application
Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 μg/mL.
Application
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 μg/mL.
Application
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 μg /ml.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DEDAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DEDAF Antibody is supplied in PBS containing 0.02% sodium azide. |
