Application
Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.Application
Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.| Product Name | DEPTOR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DEPTOR Antibody: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6 |
| Product description | DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).1) Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6. |
| Immunogen | DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DEPTOR |
|---|---|
| Alternative Names | DEPTOR Antibody: DEP.6 DEPDC6 DEP domain-containing mTOR-interacting protein DEP domain-containing protein 6 |
| Molecular Weight(MW) | Predicted: 45 kDa Observed: 42 kDa |
| Tissue Specificity | DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms. |
Application
Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.
Application
Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DEPTOR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide. |
