Application
Western blot analysis of DFF45 in HeLa cell lysate with DFF45 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DFF45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit |
| Product description | DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.1) Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 |
| Immunogen | DFF45 antibody was raised against a 20 amino acid peptide near the amino terminus of human DFF45. The immunogen is located within the first 50 amino acids of DFF45. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DFFA |
|---|---|
| Alternative Names | DFF45 Antibody: DFF1 ICAD DFF-45 DFF1 DFF45 H13 DNA fragmentation factor subunit alpha DNA fragmentation factor 45 kDa subunit |
| Molecular Weight(MW) | 45 and 35 kDa |
Application
Western blot analysis of DFF45 in HeLa cell lysate with DFF45 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DFF45 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DFF45 Antibody is supplied in PBS containing 0.02% sodium azide. |
