Application
Antibody used in WB on Human Jurkat 0.3125 ug/ml.| Product Name | DIABLO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIABLO, SMAC, DFNA64 |
| Product description | DIABLO is an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.1) Samuel, T., et al., (2006) J. Biol. Chem. 281 (2), 1080-1090. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DIABLO. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DIABLO antibody can be used for detection of DIABLO by ELISA at 1:312500. DIABLO antibody can be used for detection of DIABLO by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DIABLO |
|---|---|
| Alternative Names | DIABLO SMAC DFNA64 |
| Molecular Weight(MW) | 21 kDa, 27 kDa, 22 kDa |
Application
Antibody used in WB on Human Jurkat 0.3125 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DIABLO antibody can be used for detection of DIABLO by ELISA at 1:312500. DIABLO antibody can be used for detection of DIABLO by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DIABLO antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
