Application
Immunohistochemistry of DIS3L2 in rat brain tissue with DIS3L2 antibody at 5 μg/mL.Application
Western blot analysis of DIS3L2 in 3T3 cell lysate with DIS3L2 antibody at 1 μg/ml.| Product Name | DIS3L2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIS3 like 3'-5' exoribonuclease 2, FAM6A, PRLMNS |
| Product description | The exosome is involved in a multitude of cellular RNA processing and degradation events (1). DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2). DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3). DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4). DIS3L2 mutations have also been associated with sporadic Wilms tumors (3).1) Chen CY, Gherzi R, Ong SE, et al. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 2001; 107:451-64. |
| Immunogen | DIS3L2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DIS3L2. The immunogen is located within amino acids 720 - 770 of DIS3L2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIS3L2 |
|---|---|
| Alternative Names | DIS3 like 3'-5' exoribonuclease 2 FAM6A PRLMNS |
| Molecular Weight(MW) | Predicted: 97 kDa Observed: 94 kDa |
| Tissue Specificity | DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L. |
Application
Immunohistochemistry of DIS3L2 in rat brain tissue with DIS3L2 antibody at 5 μg/mL.
Application
Western blot analysis of DIS3L2 in 3T3 cell lysate with DIS3L2 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DIS3L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DIS3L2 antibody is supplied in PBS containing 0.02% sodium azide. |
