Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Product Name | DMAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DMAP1, EAF2, SWC4, MEAF2, DNMAP1, DNMTAP1 |
| Product description | DMAP1 is a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.1) Oh, J.H., et al., (2005) Mamm. Genome 16 (12), 942-954. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DMAP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DMAP1 antibody can be used for detection of DMAP1 by ELISA at 1:1562500. DMAP1 antibody can be used for detection of DMAP1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DMAP1 |
|---|---|
| Alternative Names | DMAP1 EAF2 SWC4 MEAF2 DNMAP1 DNMTAP1 |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DMAP1 antibody can be used for detection of DMAP1 by ELISA at 1:1562500. DMAP1 antibody can be used for detection of DMAP1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DMAP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
