Application
Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 μg/mL.Application
Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DNAL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNAL1 Antibody: CILD16, C14orf168, Dynein light chain 1, axonemal |
| Product description | DNAL1 Antibody: DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.1) Horvath J, Fliegauf M, Olbrich H, et al. Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients. Am. J. Respir. Cell Mol. Biol.2005; 33:41-7. |
| Immunogen | DNAL1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human DNAL1. The immunogen is located within the last 50 amino acids of DNAL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNAL1 |
|---|---|
| Alternative Names | DNAL1 Antibody: CILD16 C14orf168 Dynein light chain 1 axonemal |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 μg/mL.
Application
Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DNAL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DNAL1 Antibody is supplied in PBS containing 0.02% sodium azide. |
