Application
DNMT3A Antibody (Center R478WMutant) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry.Application
Western Blot at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).| Product Name | DNMT3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A |
| Product description | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].1) Holz-Schietinger, C., et al. J. Biol. Chem. 285(38):29091-29100(2010) Kelemen, L.E., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1822-1830(2010) Park, C.W., et al.Cardiovasc Transl Res 3(3):290-295(2010) Haggarty, P., et al. PLoS ONE(6), E11329 (2010)Zhao, Z., et al. J. Biomed. Biotechnol. 2010, 737535 (2010) : |
| Immunogen | This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human DNMT3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNMT3A |
|---|---|
| Alternative Names | DNA (cytosine-5)-methyltransferase 3A Dnmt3a DNA methyltransferase HsaIIIA DNA MTase HsaIIIA MHsaIIIA DNMT3A |
| Molecular Weight(MW) | 102 kDa |
Application
DNMT3A Antibody (Center R478WMutant) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry.
Application
Western Blot at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
