Application
Immunohistochemistry staining of DNMT3L in tonsil tissue using DNMT3L Antibody.| Product Name | DNMT3L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNMT3L |
| Product description | DNA cytosine-5-methyltransferase-3 proteins are involved in de novo methylation of CpG islands. Mutations in the DNMT3B gene cause the ICF immunodeficiency syndrome. DNA methyltransferases and regulatory factors are likely to be involved in the establishment of maternal imprints in growing diplotene oocytes and paternal imprints in perinatal prospermatogonia. Imprinting is essential for viable development. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. |
| Immunogen | DNMT3L antibody was raised against amino acids 322 to 337 of DNMT3L (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC |
| DNMT3L antibody can be used in ELISA, Western Blot starting at 1:1000, immunohistochemistry starting at 1.25 μg/mL, immunocytochemistry, and immunoprecipitation.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | DNMT3L |
|---|---|
| Alternative Names | DNMT3L |
Application
Immunohistochemistry staining of DNMT3L in tonsil tissue using DNMT3L Antibody.| Application Notes | DNMT3L antibody can be used in ELISA, Western Blot starting at 1:1000, immunohistochemistry starting at 1.25 μg/mL, immunocytochemistry, and immunoprecipitation.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | DNMT3L antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.1% sodium azide. |
