Application
Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.| Product Name | Dopamine Transporter Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAT, DAT1, PKDYS, Solute carrier family 6 member 3, DA transporter |
| Product description | The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).1) Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. |
| Immunogen | Peptide from the intracellular C-terminus region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| The antibody has been tested in Western blots of SDS-solubilized human and mouse striatal samples and in IHC applications with monkey (Macaque) brain sections.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC6A3 |
|---|---|
| Alternative Names | DAT DAT1 PKDYS Solute carrier family 6 member 3 DA transporter |
| Molecular Weight(MW) | 88 |
Application
Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.| Application Notes | The antibody has been tested in Western blots of SDS-solubilized human and mouse striatal samples and in IHC applications with monkey (Macaque) brain sections.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Dopamine Transporter antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
