Application
Immunofluorescence of DRAK1 in Molt cells with DRAK1 antibody at 20 μg/mL.Application
Immunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 μg/mL.Application
Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 μg/mL.| Product Name | DRAK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DRAK1 Antibody: DRAK1, DRAK1, DAP kinase-related apoptosis-inducing protein kinase 1 |
| Product description | DRAK1 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 for DAP kinase-related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK1 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK1 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.1) Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71 |
| Immunogen | DRAK1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human DRAK1. The immunogen is located within the first 50 amino acids of DRAK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DRAK1 antibody can be used for detection of DRAK1 by Western blot at 1 μg/mL. An approximately 50 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK17A |
|---|---|
| Alternative Names | DRAK1 Antibody: DRAK1 DRAK1 DAP kinase-related apoptosis-inducing protein kinase 1 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | 3) Cat. No. 17-006 - MOLT-4 Cell Slide |
| Tissue Specificity | No cross responses to DRAK2, DAP or ZIP kinases. |
Application
Immunofluorescence of DRAK1 in Molt cells with DRAK1 antibody at 20 μg/mL.
Application
Immunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 μg/mL.
Application
Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | DRAK1 antibody can be used for detection of DRAK1 by Western blot at 1 μg/mL. An approximately 50 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DRAK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DRAK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
