Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with DTX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of DTX1 Antibody in Hela cell line lysates (35ug/lane)| Product Name | DTX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase DTX1, 632-, Protein deltex-1, Deltex1, hDTX1, DTX1 |
| Product description | DTX1 was identified as encoding a positive regulator of the Notch-signaling pathway in Drosophila. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity.1) Wu,C.,et.al., Proteomics(11), 1775-1785 (2007) |
| Immunogen | This DTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the Central region of human DTX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DTX1 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase DTX1 632- Protein deltex-1 Deltex1 hDTX1 DTX1 |
| Molecular Weight(MW) | 67 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with DTX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of DTX1 Antibody in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
