Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-DUSP4 Pab in mouse brain tissue lysate (35ug/lane).Application
Western blot analysis of anti-DUSP4 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | DUSP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity protein phosphatase 4, Dual specificity protein phosphatase hVH2, Mitogen-activated protein kinase phosphatase 2, MAP kinase phosphatase 2, MKP-2, DUSP4, MKP2, VH2 |
| Product description | DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus.1) Chen, P., et al., J. Biol. Chem. 276(31):29440-29449 (2001). |
| Immunogen | This DUSP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the C-terminal region of human DUSP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | DUSP4 |
|---|---|
| Alternative Names | Dual specificity protein phosphatase 4 Dual specificity protein phosphatase hVH2 Mitogen-activated protein kinase phosphatase 2 MAP kinase phosphatase 2 MKP-2 DUSP4 MKP2 VH2 |
| Molecular Weight(MW) | 43 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-DUSP4 Pab in mouse brain tissue lysate (35ug/lane).
Application
Western blot analysis of anti-DUSP4 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
