Application
Western blot analysis of DUSP6 using rabbit polyclonal DUSP6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP6 gene.Application
Western blot analysis in CEM cell line lysates (35ug/lane).Application
Western blot analysis in mouse stomach tissue lysates (35ug/lane).| Product Name | DUSP6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity protein phosphatase 6, Dual specificity protein phosphatase PYST1, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, MKP-3, DUSP6, MKP3, PYST1 |
| Product description | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.1) Chiappinelli, K.B., et al. Gynecol. Oncol. 119(1):146-150(2010) |
| Immunogen | This DUSP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human DUSP6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DUSP6 |
|---|---|
| Alternative Names | Dual specificity protein phosphatase 6 Dual specificity protein phosphatase PYST1 Mitogen-activated protein kinase phosphatase 3 MAP kinase phosphatase 3 MKP-3 DUSP6 MKP3 PYST1 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of DUSP6 using rabbit polyclonal DUSP6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP6 gene.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Western blot analysis in mouse stomach tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
