Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-DUSP8 Pabin Hela cell line lysate (35ug/lane).| Product Name | DUSP8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity protein phosphatase 8, Dual specificity protein phosphatase hVH-5, DUSP8, C11orf81, VH5 |
| Product description | DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.1) Berger, I.R., et al., Cancer Genet. Cytogenet. 159(2):155-159 (2005). |
| Immunogen | This DUSP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-487 amino acids from the C-terminal region of human DUSP8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DUSP8 |
|---|---|
| Alternative Names | Dual specificity protein phosphatase 8 Dual specificity protein phosphatase hVH-5 DUSP8 C11orf81 VH5 |
| Molecular Weight(MW) | 66 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-DUSP8 Pabin Hela cell line lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
