Application
Antibody used in IHC on Human Heart.Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.| Product Name | DUT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DUT, FLJ20622, dUTPase |
| Product description | DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.1) Studebaker, A.W., (2005) Biochem. Biophys. Res. Commun. 327 (1), 306-310. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DUT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| DUT antibody can be used for detection of DUT by ELISA at 1:12500. DUT antibody can be used for detection of DUT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DUT |
|---|---|
| Alternative Names | DUT FLJ20622 dUTPase |
| Molecular Weight(MW) | 19 kDa, 15 kDa, 18 kDa |
| Entrez Gene | 1854 |
|---|---|
| Protein Accession | NP_001020419 |
Application
Antibody used in IHC on Human Heart.
Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DUT antibody can be used for detection of DUT by ELISA at 1:12500. DUT antibody can be used for detection of DUT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DUT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
