Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect UBE1 in HL-60 cell lysate.Application
Western blot analysis of E1 Ubiquitin (UBE1) Antibody in mouse stomach tissue lysates (35ug/lane)| Product Name | E1 Ubiquitin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1 |
| Product description | UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.1) Ayusawa, D., et al., Cell Struct. Funct. 17(2):113-122 (1992). Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88(1):258-262 (1991). Kudo, M., et al., Exp. Cell Res. 192(1):110-117 (1991). Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53(1):20-22 (1990). Zacksenhaus, E., et al., EMBO J. 9(9):2923-2929 (1990). |
| Immunogen | This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | UBA1 |
|---|---|
| Alternative Names | Ubiquitin-like modifier-activating enzyme 1 Protein A1S9 Ubiquitin-activating enzyme E1 UBA1 A1S9T UBE1 |
| Molecular Weight(MW) | 118 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect UBE1 in HL-60 cell lysate.
Application
Western blot analysis of E1 Ubiquitin (UBE1) Antibody in mouse stomach tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
