Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using E2A (Phospho-Thr355) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | E2A (phospho Thr355) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E12, Tran, TCF3, TFE2, ITF1 |
| Product description | E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.1) Kamps M.P., Cell 60:547-555(1990). |
| Immunogen | E2A (phospho Thr355) antibody was raised against a peptide sequence around phosphorylation site of threonine 355 (P-S-T (p)-P-V) derived from Human E2A . |
| Modification | p-Thr355 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCF3 |
|---|---|
| Alternative Names | E12 Tran TCF3 TFE2 ITF1 |
| Molecular Weight(MW) | 67 kDa |
| Tissue Specificity | The antibody detects endogenous levels of E2A only when phosphorylated at threonine 355. |
| Entrez Gene | 6929 |
|---|---|
| Protein Accession | NP_003191.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using E2A (Phospho-Thr355) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
