Application
Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 μg/ml.Application
Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.Application
Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.| Product Name | E2F3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F transcription factor 3, E2F-3 |
| Product description | The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).1) Lees JA, Saito M, Vidal M, et al. The retinoblastoma protein binds to a family of E2F transcription factors. Mol. Cell. Biol. 1993; 13:7813-25. |
| Immunogen | E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F3 |
|---|---|
| Alternative Names | E2F transcription factor 3 E2F-3 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 56 kDa |
| Sequence Similarities | 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
| Tissue Specificity | E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family. |
Application
Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 μg/ml.
Application
Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.
Application
Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1369 - Human Lymph node Tissue Lysate |
|---|---|
| Application Notes | E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | E2F3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | E2F3 antibody is supplied in PBS containing 0.02% sodium azide. |
