Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | E2F5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F5, E2F-5 |
| Product description | E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.1) Ohtani, N., et al., (2003) J. Cell Biol. 162 (2), 173-183. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can be used for detection of E2F5 by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F5 |
|---|---|
| Alternative Names | E2F5 E2F-5 |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can be used for detection of E2F5 by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
