Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | E2F6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F6, E2F-6 |
| Product description | E2F6 is a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants encoding several different isoforms have been found for this gene.1) Kimura, K., et al., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| E2F6 antibody can be used for detection of E2F6 by ELISA at 1:1562500. E2F6 antibody can be used for detection of E2F6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | E2F6 |
|---|---|
| Alternative Names | E2F6 E2F-6 |
| Molecular Weight(MW) | 14 kDa, 32 kDa, 18 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | E2F6 antibody can be used for detection of E2F6 by ELISA at 1:1562500. E2F6 antibody can be used for detection of E2F6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
