Application
Immunofluorescence of EED in human heart tissue with EED antibody at 20 μg/mL.Application
Western blot analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 μg/mL.| Product Name | EED Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EED Antibody: HEED, WAIT1, Polycomb protein EED, WD protein associating with integrin cytoplasmic tails 1, hEED |
| Product description | EED Antibody: The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.1) Rietzler M, Bittner M, Kolanus W, et al. The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins. J. Biol. Chem. 1998; 273:27459-66. |
| Immunogen | EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED. The immunogen is located within amino acids 30 - 80 of EED. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| EED antibody can be used for detection of EED by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EED |
|---|---|
| Alternative Names | EED Antibody: HEED WAIT1 Polycomb protein EED WD protein associating with integrin cytoplasmic tails 1 hEED |
| Molecular Weight(MW) | Predicted: 49 kDa Observed: 49 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | Four alternatively spliced transcript variants have been observed. |
Application
Immunofluorescence of EED in human heart tissue with EED antibody at 20 μg/mL.
Application
Western blot analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | EED antibody can be used for detection of EED by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EED antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EED Antibody is supplied in PBS containing 0.02% sodium azide. |
