Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Product Name | EEF1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1 |
| Product description | EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Zhang, Y., (2008) Biochem. Biophys. Res. Commun. 369 (4), 1057-1060. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EEF1A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EEF1A1 antibody can be used for detection of EEF1A1 by ELISA at 1:12500. EEF1A1 antibody can be used for detection of EEF1A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EEF1A1 |
|---|---|
| Alternative Names | EEF1A1 CCS-3 CCS3 EEF-1 EEF1A EF-Tu EF1A FLJ25721 GRAF-1EF HNGC:16303 LENG7 MGC102687 MGC131894 MGC16224 PTI1 eEF1A-1 EE1A1 |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EEF1A1 antibody can be used for detection of EEF1A1 by ELISA at 1:12500. EEF1A1 antibody can be used for detection of EEF1A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EEF1A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
