Application
With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 (1:500, 4˚C，overnight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).Application
Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of EEF1A1/ EEF1A2 Antibody in Y79, T47D cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | EEF1A1/ EEF1A2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7 |
| Product description | EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.1) Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009) |
| Immunogen | This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EEF1A1 |
|---|---|
| Alternative Names | Elongation factor 1-alpha 1 EF-1-alpha-1 Elongation factor Tu EF-Tu Eukaryotic elongation factor 1 A-1 eEF1A-1 Leukocyte receptor cluster member 7 EEF1A1 EEF1A EF1A LENG7 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 (1:500, 4˚C，overnight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).
Application
Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of EEF1A1/ EEF1A2 Antibody in Y79, T47D cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
