Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Ab-1070). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using EGFR (Ab-1070).Application
Western blot analysis of lysed extracts from HUVEC and MDA cells using EGFR (Ab-1070).| Product Name | EGFR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB, HER1, mENA, ERBB1, PIG61, GLUH1, GLUR1, Receptor tyrosine-protein kinase ErbB-1 |
| Product description | Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia1) Feinmesser RL, et al. (1999) J Biol Chem; 274 (23): 16168-73. Gamou S, et al. (1995) FEBS Lett; 357 (2): 161-4. Gamou S, et al. (1994) J Cell Physiol; 158 (1): 151-9. Heisermann GJ, et al. (1988) J Biol Chem; 263 (26): 13152-8. |
| Immunogen | EGFR (Ab-1070) antibody was raised against a peptide sequence around aa.1068~1072 (R-Y-S-S-D) derived from Human EGFR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGFR |
|---|---|
| Alternative Names | ERBB HER1 mENA ERBB1 PIG61 GLUH1 GLUR1 Receptor tyrosine-protein kinase ErbB-1 |
| Molecular Weight(MW) | 175 kDa |
| Tissue Specificity | This antibody detects endogenous level of total EGFR protein. |
| Entrez Gene | 1956 |
|---|---|
| Protein Accession | NP_005219.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Ab-1070). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using EGFR (Ab-1070).
Application
Western blot analysis of lysed extracts from HUVEC and MDA cells using EGFR (Ab-1070).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
