Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1197).Application
Western blot analysis of lysed extracts from HUVEC cells untreated or treated with EGF using EGFR (Phospho-Tyr1197).| Product Name | EGFR (phospho Tyr1197) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB, HER1, mENA, ERBB1, PIG61, MADH2, MADR2, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR |
| Product description | Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.1) Corbalan-Garcia S, et al. (1996) Mol Cell Biolt; 16 (10): 5674-5682Kanner SB, et al. (1991) Mol Cell Biol; 11 (2): 713-720Wu TT, et al. (1998) Mol Biol Cell; 9 (7): 1661-1674O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94 (7): 3250-3255Goldstein DJ, et al. (1994) J Virol; 68 (7): 4432-4441 |
| Immunogen | EGFR (Phospho-Tyr1197) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y (p) -L-R) derived from Human EGFR. |
| Modification | p-Tyr1197 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGFR |
|---|---|
| Alternative Names | ERBB HER1 mENA ERBB1 PIG61 MADH2 MADR2 Receptor protein-tyrosine kinase ErbB-1 kinase EGFR |
| Molecular Weight(MW) | 175 kDa |
| Tissue Specificity | This antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1197. |
| Entrez Gene | 1956 |
|---|---|
| Protein Accession | NP_005219.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1197).
Application
Western blot analysis of lysed extracts from HUVEC cells untreated or treated with EGF using EGFR (Phospho-Tyr1197).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
