Application
Western blot analysis of lysates from A431, HUVEC cell line (from left to right), using EGFR Antibody at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded H. skin section using EGFR Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Product Name | EGFR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1 |
| Product description | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.1) Abdallah, R.T., et al. J. Biol. Chem. 285(45):35206-35215(2010) |
| Immunogen | This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the N-terminal region of human EGFR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGFR |
|---|---|
| Alternative Names | Epidermal growth factor receptor Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1 EGFR ERBB ERBB1 HER1 |
| Molecular Weight(MW) | 134 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of lysates from A431, HUVEC cell line (from left to right), using EGFR Antibody at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded H. skin section using EGFR Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
