Application
Western blot analysis of EGFR using rabbit polyclonal EGFR Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EGFR gene (Lane 2).Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | EGFR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1 |
| Product description | EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.1) Aifa, S., et al., Exp. Cell Res. 302(1):108-114 (2005). |
| Immunogen | This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 847-876 amino acids from human EGFR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGFR |
|---|---|
| Alternative Names | Epidermal growth factor receptor Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1 EGFR ERBB ERBB1 HER1 |
| Molecular Weight(MW) | 134 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of EGFR using rabbit polyclonal EGFR Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EGFR gene (Lane 2).
Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
