Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EGFR Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | EGFR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1 |
| Product description | EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.1) Aifa, S., et al., Exp. Cell Res. 302(1):108-114 (2005). |
| Immunogen | This EGFR antibody is generated from rabbits immunized with EGFR his fusion protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EGFR |
|---|---|
| Alternative Names | Epidermal growth factor receptor Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1 EGFR ERBB ERBB1 HER1 |
| Molecular Weight(MW) | 134 kDa |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EGFR Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
