Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Product Name | EGLN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EGLN2, EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1 |
| Product description | The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EGLN2 antibody can be used for detection of EGLN2 by ELISA at 1:62500. EGLN2 antibody can be used for detection of EGLN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN2 |
|---|---|
| Alternative Names | EGLN2 EIT6 PHD1 HPH-1 HPH-3 HIFPH1 HIF-PH1 |
| Molecular Weight(MW) | 16 kDa |
Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | EGLN2 antibody can be used for detection of EGLN2 by ELISA at 1:62500. EGLN2 antibody can be used for detection of EGLN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EGLN2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
