Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with EGLN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of EGLN2 Antibody in MDA-MB231, Hela cell line lysates (35ug/lane)| Product Name | EGLN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Egl nine homolog 2, Estrogen-induced tag 6, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 1, HIF-PH1, HIF-prolyl hydroxylase 1, HPH-1, Prolyl hydroxylase domain-containing protein 1, PHD1, EGLN2, EIT6 |
| Product description | EGLN2 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.1) Semenza,G.L. et.al., Cell 107 (1), 1-3 (2001) Aprelikova,O., et.al., Cancer Res. 69 (2), 616-624 (2009) |
| Immunogen | This EGLN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-221 amino acids from the Central region of human EGLN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN2 |
|---|---|
| Alternative Names | Egl nine homolog 2 Estrogen-induced tag 6 HPH-3 Hypoxia-inducible factor prolyl hydroxylase 1 HIF-PH1 HIF-prolyl hydroxylase 1 HPH-1 Prolyl hydroxylase domain-containing protein 1 PHD1 EGLN2 EIT6 |
| Molecular Weight(MW) | 44 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with EGLN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of EGLN2 Antibody in MDA-MB231, Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
