Application
Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using eIF2α (Phospho-Ser51).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using eIF2α(Phospho-Ser51).| Product Name | eIF2a (phospho Ser51) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, STK14A, Eukaryotic translation initiation factor 2 subunit alpha |
| Product description | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.1) Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460. Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992. Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965. |
| Immunogen | eIF2a (Phospho-Ser51) antibody was raised against a peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2α. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2S1 |
|---|---|
| Alternative Names | EIF2 EIF-2 EIF2A EIF-2A EIF-2alpha STK14A Eukaryotic translation initiation factor 2 subunit alpha |
| Molecular Weight(MW) | 38 kDa |
| Tissue Specificity | This antibody detects endogenous level of eIF2α only when phosphorylated at serine 51. |
| Entrez Gene | 1965 |
|---|---|
| Protein Accession | NP_004085.1 |
Application
Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using eIF2α (Phospho-Ser51).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using eIF2α(Phospho-Ser51).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM277741