Application
EIF2AK3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | EIF2AK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK |
| Product description | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.1) Xu, H., et al. Toxicology 277 (1-3), 1-5 (2010) : |
| Immunogen | This EIF2AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human EIF2AK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK3 |
|---|---|
| Alternative Names | Eukaryotic translation initiation factor 2-alpha kinase 3 PRKR-like endoplasmic reticulum kinase Pancreatic eIF2-alpha kinase HsPEK EIF2AK3 PEK PERK |
| Molecular Weight(MW) | 125 kDa |
Application
EIF2AK3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
