Application
Immunohistochemistry staining of EIF2S1 in placenta using EIF2S1 Antibody.Application
Immunohistochemistry staining of EIF2S1 in adrenal tissue using EIF2S1 Antibody.| Product Name | EIF2S1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF2S1, EIF-2-alpha, EIF-2alpha, EIF2 alpha, EIF2, EIF-2, EIF-2A |
| Product description | eIF2 is a translation initiation factor that catalyzes the first regulated step of protein synthesis, promoting the binding of the initiator tRNA to 40s ribosomal subunits. eIF2 is composed of 3 non identical subunits, alpha, beta and gamma. Binding occurs as a ternary complex of methionyl-tRNA, eIF2 and GTP. The rate of complex formation is modulated by the phosphorylation state of eIF2alpha. Phosphorylation of eIF2alpha has been shown to occur in response to heat shock and hypoxic stress. Once eIF2alpha is phosphorylated, the initiation of protein synthesis is inhibited. Expression of eIF-2alpha has been shown to increase in melanoma and colon carcinoma. |
| Immunogen | EIF2S1 antibody was raised against amino acids surrounding Ser 51 of EIF2S1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| EImmunofluorescence2S1 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:200, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2S1 |
|---|---|
| Alternative Names | EIF2S1 EIF-2-alpha EIF-2alpha EIF2 alpha EIF2 EIF-2 EIF-2A |
| Tissue Specificity | pSer51 |
Application
Immunohistochemistry staining of EIF2S1 in placenta using EIF2S1 Antibody.
Application
Immunohistochemistry staining of EIF2S1 in adrenal tissue using EIF2S1 Antibody.| Application Notes | EImmunofluorescence2S1 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:200, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store EIF2S1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.02% sodium azide, 50% glycerol. |
