Application
Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).| Product Name | eIF4B (phospho Ser422) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF-4B, PRO1843, ESR, NR3A1, |
| Product description | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.1) Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. and Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481. |
| Immunogen | eIF4B (phospho-Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (T-G-S (p) -E-S) derived from Human eIF4B. |
| Modification | p-Ser422 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4B |
|---|---|
| Alternative Names | EIF-4B PRO1843 ESR NR3A1 |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | This antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422. |
| Entrez Gene | 1975 |
|---|---|
| Protein Accession | NP_001408.2 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
